Fig. 3: EG7 and human CRC CAFs share similar transcriptome profiles. | Nature Communications

Fig. 3: EG7 and human CRC CAFs share similar transcriptome profiles.

From: CD73 on cancer-associated fibroblasts enhanced by the A2B-mediated feedforward circuit enforces an immune checkpoint

Fig. 3: EG7 and human CRC CAFs share similar transcriptome profiles.The alternative text for this image may have been generated using AI.

a Comparative expression profile of CAFs and MSCs was analyzed via RNA sequencing. The volcano plot illustrates genes that were significantly upregulated (995, red) and downregulated (589, blue) adjusted for fold change > 2 and p < 0.01 with representative genes associated with specific pathways. b Gene sets specifically enriched among the differentially expressed genes in CAFs were analyzed via gene set enrichment analysis (GSEA). c–f The 417 genes categorized in “response to stimulus” were further analyzed for enrichment in KEGG pathways. Representative heatmaps of RNA sequencing data with genes significantly up-regulated in CAFs according to specific pathways were shown from three biological repeats, each extracted from at least five pooled tumor-bearing mice. g Gene set enrichment analysis (GSEA) of differentially expressed genes in human CRC-CAFs compared with paired tumor cells (GSE39396) identified highly enriched genes in pathways related to cytokine–cytokine interaction and ECM receptor interaction. h The gene expression profile heatmap shows differentially expressed genes in paired human CRC tumors, TILs, and CAFs (GSE39396) associated with pathways highly enriched in CRC-CAFs. The list on the right showed a selective representative shortlist of significantly up-regulated genes in CAFs, most of which are similar to those identified in EG7-CAFs. Source data are provided in the Source Data file.

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