Fig. 4: AZD1480 treatment reduced the infiltration of CD11b + myeloid cells associated with sevoflurane in the lung. | Nature Communications

Fig. 4: AZD1480 treatment reduced the infiltration of CD11b + myeloid cells associated with sevoflurane in the lung.

From: Distinct effects of general anesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models

Fig. 4

a Sevoflurane increased CD11b+ cells in the lung one day after surgery compared to propofol by immunofluorescence imaging. Treatment with AZD1480 significantly reduced infiltrated CD11b+ cells (green) induced by sevoflurane in the lungs (n = 4 mice in each group; sevoflurane vs propofol, p = 0.007; sevoflurane vehicle vs AZD1480, p = 0.032; two-way ANOVA + Dunnett’s post hoc tests, Scale bar = 20 μm). No significant change was observed with CD31 positive cells (red). One day surgery, lungs of the mice were sectioned and stained with anti-CD11b, anti-CD31, and DAPI. b One day after surgery, AZD1480 treatment dramatically reduced serum and lung tissue level of IL-6 (n = 4, p = 0.001 and 0.02 respectively, two-way ANOVA + Dunnett’s post hoc tests) by ELISA assays. c Western blot analysis of STAT3 phosphorylation showed that AZD1480 treatment significantly reduced p-Tyr705 (sevoflurane vs propofol p = 0.03, sevoflurane vs AZD1480 p = 0.001, two-way ANOVA + Dunnett’s post hoc tests) and p-Ser727 (sevoflurane vs AZD1480 p = 0.001, n = 4, two-way ANOVA + Dunnett’s post hoc tests) one day after surgery with sevoflurane. Data presented as the mean ± S.D. Source data are provided as a Source Data file.

Back to article page