Fig. 3: Mast cell-specific Tph1 deletion protects HFD-fed mice from obesity and insulin resistance. | Nature Communications

Fig. 3: Mast cell-specific Tph1 deletion protects HFD-fed mice from obesity and insulin resistance.

From: Genetic deletion of mast cell serotonin synthesis prevents the development of obesity and insulin resistance

Fig. 3

a Breeding strategy to produce Carboxypeptidase-3 (Cpa3) Cre Tph1 double floxed mice. b Weight and c Fat mass over time (n = 12). d WAT mass % of body weight (n = 12). e Glucose tolerance test (GTT), 1.5 g/kg glucose (n = 12). f Insulin tolerance test (ITT), 1 U/kg insulin (n = 12). g Overnight 12 h fasted blood glucose (BG, n = 12). h Liver weight in grams (n = 12). i mg of Triglycerides (TG) per gram of liver tissue (n = 12). j Representative liver H & E images, scale bar 100 µm. k Serotonin concentration in platelet poor plasma (PPP) samples (n = 8 Tph1fl/fl, 7 Tph1 MCKO). Statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) determined by two-way RM ANOVA with Bonferroni post-test or Student’s t-test. All data are expressed as mean ± SEM.

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