Fig. 4: Genetic removal of mast cell serotonin increases energy expenditure and causes adipose tissue browning.

a Resting oxygen consumption of C57BL6J (n = 12), Kit^Sham (n = 13), Kit^Tph+/+ (n = 11) and Kit^Tph−/− (n = 14). b Resting oxygen consumption of Tph1 MCKO mice (n = 12). c Tph1 expression in iWAT of C57BL6J (n = 13), Kit^Sham (n = 8), Kit^Tph+/+ (n = 9) and Kit^Tph−/− (n = 8). d Adipose tissue serotonin (5HT) of C57BL6J (n = 13), Kit^Sham (n = 12), Kit^Tph+/+ (n = 11) and Kit^Tph−/− (n = 13). e Ucp1 expression of C57BL6J (n = 15), Kit^Sham (n = 14), Kit^Tph+/+ (n = 12) and Kit^Tph−/− (n = 16). f Tph1 expression in WAT of Tph1 MCKO mice (n = 12). g Representative images of H & E and Ucp1 IHC-stained iWAT of Tph1 MCKO mice, Scale bar 100 µm. h Ucp1 expression in iWAT from Tph1 MCKO mice (n = 12). i Representative images of H & E and Ucp1 IHC-stained gWAT of Tph1 MCKO model, Scale bar 100 µm. j Ucp1 expression of gWAT from Tph1 MCKO model (n = 12). Statistical significant effects (*p < 0.05, **p < 0.01 and ****p < 0.0001) determined by one-way ANOVA with uncorrected Fisher’s LSD post-test or Student’s t-test where appropriate. All data are expressed as mean ± SEM.