Fig. 5: Wag31 relocalizes from the old to the new pole during the pre-NETO phase.
From: A biphasic growth model for cell pole elongation in mycobacteria
a Combined time-lapse phase-contrast and fluorescence microscopy of M. smegmatis expressing Wag31-GFP. Zoom on the division site showing progressive formation of two elongating new poles. Time between consecutive images is 20 min. Scale bar, 1 μm. b New-pole elongation curves of two sibling cells (red and orange) and the average of their Wag31-GFP intensities (green). Data are representative of 20 poles (Supplementary Fig. 7). The average intensity rather than individual intensity of the two new poles is plotted because their physical proximity precluded individual measurements. Wag31-GFP intensity over time was fitted with an asymptotic exponential function (dotted green line) with a time constant of 1.9 h (confidence interval 0.26 at 95%), adjusted R2 = 0.98. The confidence interval and R2 value indicate the quality of the fit to the fluorescence curve. c Combined time-lapse fluorescence and atomic force microscopy of M. smegmatis expressing Wag31-Dendra2 before (0–), just after (0+), and 2 h 51 min after (2h51) UV-induced photo-conversion. Scale bar, 1 μm. d Evolution of photo-converted Wag31-Dendra2 signal at the new and old cell poles during cell division. Each sibling cell has a new pole (orange) and an old pole (light or dark blue) inherited from the mother cell. e Schematic representation of Wag31 relocalization from the old pole to the new pole during and after cell division.
