Fig. 5: ICP27 is a sequence-dependent activator of mRNA 3′ processing.
From: Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27
The core sequences (−100 nt to +100 nt (a)) or the extended sequences (core plus 1440 nt upstream sequences (b)) of four PASs, including SPTSSA and KDM4C (showed DoTT in HSV-1-infected cells) and POLR2A and ICP27 (no DoTT), were cloned into pPASPORT. These reporters were co-transfected with vector or an ICP27-expressing plasmid and mRNA 3′ processing activities (Rluc/Fluc) were plotted as mean ± s.d. c The core and extended PAS of KDM4C and ICP27 as well the chimeric PASs between the two PASs were co-transfected with vector or an ICP27-expressing plasmid and the PAS activities (Rluc/Fluc) were plotted as mean ± s.d. The GC contents of the upstream sequences of both PASs are marked on the left. d The GC content at HSV-1 and human PASs. e The GC contents of PASs of host genes with significant DoTT (red) and without DoTT (green). Reporter assays in a, b, and c were performed at the same time under the same conditions and were displayed in multiple panels for comparisons.
