Fig. 8: Bach2 limits DNA accessibility, occupancy and expression of IRF4 target genes.

a Bach2 and IRF4 ChIP-seq tracks showing sites of Bach2 and IRF4 co-binding within the Il10 and Il2ra loci of naïve splenic CD4 T cells cultured in Treg cell-inducing conditions. Boxes indicate Bach2 binding sites with increased IRF4 occupancy in the absence of Bach2. b Quantification of IRF4 and Bach2 co-binding. c Frequencies of differentially expressed genes bound by both Bach2 and IRF4 that are upregulated in the absence of Bach2. d ATAC-seq of naïve splenic CD4 T cells from Cd4^Cre or Bach2^fl/flCd4^Cre mice before and after 72 h culture in Treg cell-inducing conditions. Heatmap showing Z-scores of read densities at peaks called differentially accessible between genotypes that also co-localize with Bach2 ChIP-seq peaks. e Example loci showing ATAC-seq, IRF4 ChIP-seq and Bach2 ChIP-seq tracks. Boxes indicate Bach2 binding sites with increased IRF4 occupancy and chromatin accessibility in the absence of Bach2. f Number of IRF4 ChIP-seq peaks called in wildtype or Bach2^−/− cells at sites of differentially accessible chromatin. Source data are provided as a Source Data file.