Fig. 4: Induction and secretion of pseudoalterin in strain CF6-2.

a Induction of psn expression in strain CF6-2 by PG of strain MCCC0423. Control, strain CF6-2 was cultured in the medium without PG. b Inducing effects of peptides derived from PG on psn expression. O, l-Orn; iq, d-iso-Gln. c Inducing effects of amino acids from PG on psn expression. d Inducing effects of different concentrations of glycine on psn expression. e The extracellular pseudoalterin production of strain CF6-2 induced by 2 mM glycine added at 0, 6, and/or 12 h. Control, strain CF6-2 was cultured in the medium without glycine. The pseudoalterin production was represented by the extracellular elastinolytic activity. f Genetic organization of the T2SS cluster of strain CF6-2. Each gene is represented by an arrow. The “core” genes in the cluster are marked in orange and the others (gspA, B, and N) in blue. g Pseudoalterin production of strain CF6-2 and its mutants cultured in a fermentation medium. The upper shows pseudoalterin production represented by extracellular elastinolytic activity. The lower shows pseudoalterin production detected by western blot. h Non-contact co-culture of strain ΔgspE and strain MCCC0423. The upper panel shows the growth of the strains. The lower panel shows pseudoalterin production detected by western blot. The graphs show data from triplicate experiments (mean ± SD). Source data are provided as a Source Data file.