Fig. 4: Large IncHI2 plasmid with ~55 kb multidrug resistance region containing blaIMP-4. | Nature Communications

Fig. 4: Large IncHI2 plasmid with ~55 kb multidrug resistance region containing blaIMP-4.

From: Integrating multiple genomic technologies to investigate an outbreak of carbapenemase-producing Enterobacter hormaechei

Fig. 4

a A 330,060 bp IncHI2 plasmid carrying multiple resistance operons, including a large ~55 kb multidrug resistance (MDR) region, was fully recovered and assembled using Pacific Biosciences (PacBio) SMRT sequencing of strain MS7884 (patient 1, isolate 1). The multidrug resistance region was found to contain two class 1 integrons (In809, In37) along with several other antibiotic resistance genes, as indicated. Comparison of this MDR region to publicly available genomes found a close match to pEl1573, isolated in 2012 from an E. cloacae isolate in Sydney, Australia. b A predicted model of homologous recombination between two nearly identical aacA4/aac(6′)-Ib-cr genes (red asterisks) within the ~55 kb MDR region in MS7889 (patient 2, isolate 4, IMP-, carbapenem-susceptible) leading to the loss of a ~34 kb region containing blaIMP-4 as well as several other antibiotic resistance genes.

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