Fig. 2: Validation of 4C interaction sites by FISH.
From: Epigenetic specifications of host chromosome docking sites for latent Epstein-Barr virus
a Positions of FISH probes (green bars) on chr2, 4, 12, and 17. b Precise positions of FISH probes (green bars) on chr2 with common 4C peaks. c Representative images for interphase MutuI nuclei stained with dual FISH for EBV-488 (Oregon Green 488) and Target-594 (Alexa Fluor 594). d Representative images of interphase MutuI nucleus stained with dual FISH for EBV-488 and Target-594. e, f The percentage of co-localized FISH signals between EBV-488 and Target-594 in interphase nuclei from MutuI (panel e) and Raji (panel f) cells. The FISH signals were measured in more than 100 nuclei. g Representative images of mitotic MutuI nuclei stained with dual FISH for EBV-488 and Target-594. All arrows indicate co-localized FISH signals between EBV-488 and Target-594. h The percentage of co-localized FISH signals between EBV-488 and Target-594 in mitotic chromosomes from MutuI cells. The FISH signals were measured in more than 50 nuclei. Error bar represents the standard deviation and P-value were calculated by two-sided Student t-test.
