Fig. 6: TGF-β1 expressing macrophages are present during HO formation.
From: Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing
a Left: GSEA analysis of microarray data collected from buffy coat of human burn injury patients at increased risk of HO compared to post-surgical control patients. Right: GSEA analysis of RNAseq performed of tendon injury site 3 weeks after burn/tenotomy in mice. b Western blot of whole tissue protein collected from the injury site of C57BL/6 J mice after burn/tenotomy at indicated time points. A western blot for p-SMAD2 and H3 was performed on the nuclear fraction and SMAD2 and alpha-Tubulin were performed on the cytosolic fraction. n = 5 were pooled for each time point. c Co-localization of F4/80+ and TGF-β1 at tendon injury site 1 week after burn/tenotomy. Scale bars correspond to 100 μm. d Left: Co-localization of CD68+ and TGF-β1 in early human HO. Right: Co-localization of p-SMAD2 and PDGFRα in human HO. Source data are provided as a Source Data file.
