Fig. 2: Lack of β4GalT1 leads to a perturbed HSC phenotype.

Phenotypic analysis of a Ter-119⁺, CD3⁺, B220⁺, and CD11b⁺. n = 5 in each group. Data are expressed as mean ± SEM. Groups were compared using an unpaired Student’s t-test, *p < 0.05. b LT-HSCs, ST-HSCs, and MPPs (n = 4 in each group), and c CMPs, GMPs, PreMegEs, and MKPs cells (n = 4 in each group), and d MKs (n = 3 in each group) in BMs of control (white) and B4galt1^−/− (green) mice. The molecular markers utilized to identify specific subpopulations of hematopoietic stem and progenitor cells are summarized in Supplementary Table 1. Data are expressed as mean ± SEM. Groups were compared using an unpaired Student’s t-test, *p < 0.05; **p < 0.01. e Colony forming units obtained and quantified from BM HSCs isolated from control (white) and B4galt1^−/− (green) mice BMs. n = 5 in each group. Data are expressed as mean ± SEM. Groups were compared using an unpaired Student’s t-test, *p < 0.05. f Quantification of cells in G0, G1, and G2/M phase of the cell cycle within the LT-HSC compartment (n = 4 in each group), and g CD41⁺ LT- and ST-HSCs (n = 5 in each group) in control (white) and B4galt1^−/− (green) mice by flow cytometry. Data are expressed as mean ± SEM. Groups were compared using an unpaired Student’s t-test, n.s. = not significant; **p < 0.01. h Flow cytometry plots showing the gating strategy to identify LT-HSC and the CD41⁺ subset in control and B4galt1^−/− BM cells. Lineage negative cells were plotted for Sca and Kit. The double positive population (LSK) was further plotted for CD150 and CD48. LT-HSCs were gated as CD150⁺ and CD48⁻ and evaluated for CD41 expression. i Quantification of TPO, PDGF, FGF-1, TGFβ1, TNFα, IFNβ, and IL-6 in the BM supernatant of control (white) and B4galt1^−/− (green) mice. n ≥ 4 per group. Data are expressed as mean ± SEM. Groups were compared using an unpaired Student’s t-test, **p < 0.01.