Table 2 Hematologic profiling of blood and bone marrows in WT, B4galt1^−/−, Itgb1^PF4+, and B4galt1^−/−Itgb1^PF4+ mice.

Parameter	WT	B4galt1^−/−	Itgb1^PF4+	B4galt1^−/−Itgb1^PF4+
Peripheral blood cells (6–8 weeks)
RBCs (10⁶/µl)	8.6 ± 0.9	7.7 ± 0.5	8.1 ± 0.5	6.5 ± 0.6*
Neutrophils (K/µl)	0.9 ± 0.1	4.0 ± 0.7***	0.6 ± 0.1	3.5 ± 1.0**
Lymphocytes (K/µl)	8.6 ± 0.8	10.2 ± 1.3	7.4 ± 0.8	11.4 ± 2.7
Monocytes (K/µl)	1.5 ± 0.2	2.5 ± 0.5	1.7 ± 0.3	3.5 ± 0.8*
Platelets (K/µl)	893 ± 36	199 ± 39***	990 ± 9	551 ± 29***
Platelet half-life (h)	63.2 ± 2	66.7 ± 5	n.a.	n.a.
Plasma TPO (pg/ml)	87.9 ± 5.5	328.5 ± 28.8***	n.a	n.a
Spleen
mg/g	3.4 ± 0.2	6.9 ± 1.1**	n.a.	n.a.
Megakaryocytes/field	1.4 ± 0.2	4 ± 0.8**	n.a.	n.a.
LT-HSC (%)	0.0018 ± 0.0007	0.006 ± 0.003	n.a.	n.a.
ST-HSC	0.003 ± 0.001	0.003 ± 0.001	n.a.	n.a.
MPP	0.03 ± 0.006	0.16 ± 0.09	n.a.	n.a.
Bone marrow megkaryocytes
MK (% of live cells)	1.5 ± 0.1	5.6 ± 1.4*
GPIbα (MFI)	308.3 ± 24.6	244.4 ± 46.0	n.a.	n.a.
GPIbβ (MFI)	316.4 ± 5.1	319.0 ± 10.8	n.a.	n.a.
GPIX (MFI)	322.1 ± 10.2	326.8 ± 13.9	n.a.	n.a.
αIIb (MFI)	326.0 ± 46.2	298.5 ± 37.7	n.a.	n.a.
β3 (MFI)	145.1 ± 11.4	165.4 ± 30.1	n.a.	n.a.
β1 (MFI)	467.3 ± 34.6	581.2 ± 25.5*	n.a.	n.a.
CXCR4 (MFI)	135.9 ± 12.05	159.2 ± 17.34	n.a.	n.a.
Primary transplant (% GFP⁺ bone marrow cells)
MK	0.07 ± 0.002	0.4 ± 0.08*	n.a.	n.a.
CD3+	2.6 ± 0.3	0.8 ± 0.2**	n.a.	n.a.
B220+	11.6 ± 1.7	0.8 ± 0.2***	n.a.	n.a.
CD11b+	80.2 ± 2.9	95.2 ± 0.8**	n.a.	n.a.
GPIbα (BM MKs, MFI)	339.8 ± 31.2	317.7 ± 37.6	n.a.	n.a.
β₃ (BM MKs, MFI)	160.3 ± 1.8	173.4 ± 2.6	n.a.	n.a.

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