Fig. 4: Thermal denaturation and kinetics of folding and unfolding of a DNA triplex.
From: Studying biomolecular folding and binding using temperature-jump mass spectrometry
a Representation of the chemical equilibrium of formation of a DNA triplex. b Thermal denaturation experiment of a DNA triplex monitored by native MS (10 µM Ma, 10 µM Mb, and 30 µM Mc in 100 mM TMAA at pH 5.5). c–e Kinetics of triplex formation monitored using T-jump ESI-MS, using jumps from from 60 to 20 °C, 25–35 °C, and 35–10 °C, as indicated. For the kinetics, a model corresponding to the chemical equations displayed in a was fitted to the data to obtain the rate constants. f Van’t Hoff plots for the formation of the duplex and the triplex. The intercept is proportional to ∆S0 and the slope to ∆H0. g Eyring plot for the formation of the duplex and the formation and dissociation of the triplex. The intercept is proportional to ∆S‡ and the slope to ∆H‡.
