Fig. 6: Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice. | Nature Communications

Fig. 6: Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.

From: TRIM25 promotes the cell survival and growth of hepatocellular carcinoma through targeting Keap1-Nrf2 pathway

Fig. 6

a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 and shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) and representative image (b) and weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining and the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 and Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) and statistical data (h) of TRIM25, Keap1 and Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 and TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, and h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests.

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