Fig. 3: Structural details of BG505 F14 SOSIP and BG505 F14/Vt8 SOSIP.
From: Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
a Refined cryo-EM map of BG505 F14 SOSIP (gp120 in blue and gp41 in orange) bound to VRC01 (purple). b Refined cryo-EM map of BG505 F14/Vt8 SOSIP (gp120 in blue and gp41 in orange) bound to VRC03 (red) and 10-1074 (green). c Alignment of BG505 F14 SOSIP gp140 and BG505 F14/Vt8 SOSIP gp140. RMSD is for the alignment of gp120 and gp41 together. d Cryo-EM map of the BG505 F14/Vt8 construct with fitted coordinates depicting the F14 mutation sites. e Alignment of the BG505 F14/Vt8 SOSIP gp120 with the BG505 SOSIP gp120 showing the relative positions of the F14 mutations. f Cryo-EM map of the BG505 F14/Vt8 construct with fitted coordinates depicting the Vt8 mutations. g Alignment of the BG505 F14/Vt8 SOSIP gp120 with the BG505 SOSIP gp120 showing the relative positions of the Vt8 mutation sites. h, i Transparent cryo-EM maps with ribbon representations of refined coordinates for BG505 F14 and F14/Vt8 SOSIPs (gp120 in blue and gp41 in orange) identifying gp41 HR1 helical extension. j Alignment of gp41 three-helix bundle helix (residues 571-596) of BG505 F14 and F14/Vt8 SOSIPs (light orange and orange, respectively), an open state BG505 SOSIP (PDB ID 5VN3; green), and a closed state BG505 SOSIP (PDB ID 5CEZ; blue) identifying the extension of the gp41 HR1.
