Fig. 2: PDLP5 localizes to plasmodesmata in LRP-overlying cells.

a Representative confocal images showing 2D maximum intensity projections of 10 μm-thick cross-sections of PDLP5pro:PDLP5-GFP. Plasmodesmata marked by punctate PDLP5-GFP signal (in cartoons, red dots) can be seen at the cell junctions. PDLP5-GFP signals vary in color ranging from blue to green, yellow, and white depending on fluorescence intensity as indicated in calibration bar of the fluorescence intensity included on the right. The dashed arcs are positions of LRP; open arrowheads, separated cell walls; carets, strong PDLP5-GFP signal in cell walls just prior to separation. Scale bars, 25 μm. b A representative 3-D rendering of a confocal image, showing a 2-D maximum intensity projection of a 45 μm-thick cross-section of cells overlying an emerged LRP (not rendered) in PDLP5pro:PDLP5-GFP root. Signals at other junctions (Co–Co, Ep–Ep) were rare at the emergence stage we used for modeling. Inset, a cross-sectional view of the image taken from the boxed area. PDLP5-GFP-labeled plasmodesmata in different cell junctions are color-coded. c pER8:PDLP5 root tips stained with aniline blue after 24 h of 10 µM estradiol or mock treatment. Estradiol- and mock-treated n = 25; scale bars, 25 µm. d PDLP5 induction can prevent GFP movement from En to Co root tissue in pER8:PDLP5 × CASP1:GFP F2 crosses. Mock n = 24, Treated n = 47; scale bars, 20 μm.