Fig. 1: Early-life microbiota regulates Erdr1 expression. | Nature Communications

Fig. 1: Early-life microbiota regulates Erdr1 expression.

From: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

Fig. 1: Early-life microbiota regulates Erdr1 expression.The alternative text for this image may have been generated using AI.

a Experimental schematic of the exGF model: Germ-free (GF) mice were colonized with microbiota beginning at day 21 by co-housing with SPF mice for 3 weeks. b Volcano plots showing log2 fold-change of total colon gene expression in SPF mice compared with exGF or GF mice. Genes that were significantly decreased in exGF or GF compared with SPF colons are highlighted on red, while those increased in exGF or GF are highlighted in blue. c qPCR analysis of Erdr1 using whole small and large intestinal tissue from SPF, exGF, and GF mice. n = 2 (duodenum/jejunum/ileum from exGF), n = 4 (duodenum/jejunum/ileum from SPF and GF, colon). d ChIP analysis for histone H3 acetylation (H3Ac) in the Erdr1 promoter region using total colon tissue. n = 4; two independent experiments. All data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s multiple comparison test.

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