Fig. 3: Erdr1 increases growth of intestinal organoids. | Nature Communications

Fig. 3: Erdr1 increases growth of intestinal organoids.

From: Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

Fig. 3: Erdr1 increases growth of intestinal organoids.The alternative text for this image may have been generated using AI.

a Expression of Erdr1 in intestinal small and large organoids derived from SPF, exGF and GF mice. n = 4. b ChIP analysis for histone H3 acetylation (H3Ac) in the Erdr1 promoter region using large intestine (LI) organoids derived from SPF, exGF, and GF mice. n = 4; two independent experiments. c Representative images of LI organoids derived from SPF, exGF, and GF mice and cultured ±Erdr1 (1 μg) for 6 days. d Organoid efficiency of LI organoids cultured ±Erdr1. n = 4; representative of three experiments. e Frequency of budding organoids cultured ±Erdr1 for 6 days. n = 4; representative of three experiments. f Surface area of LI organoids cultured ±Erdr1. n = 21; representative of three experiments. All data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired, two-tailed t-tests.

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