Fig. 4: OsTb23bp+ negatively regulates D14 via the OsTb1-OsMADS57-D14 pathway.
From: Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice
a Effects of OsTb23bp+ and OsTb23bp− on the transcriptional regulation of D14 in rice protoplasts. b Effects of OsTb23bp+ on the transcriptional regulation of D14 via the OsTb1-OsMADS57-D14 pathway in Nicotiana benthamiana. OsGRF4 is used as a negative control for detecting the effects of OsTb2 on D14pro::LUC activity. Each relative luciferase activity of D14pro::LUC value in a and b represents the mean ± s.d. (n = 10 biologically independent samples). Student’s t-test analysis indicated a significant difference (*P < 0.05, **P < 0.01). c In the yeast one-hybrid assay, OsTb2 fusion proteins fail to activate D14pro::LacZ reporter gene expression in yeast. The right diagrammatic drawing indicates the yeast strains transformed with related plasmids. d A schematic model depicts that OsTb2 interacts with OsTb1 to regulate the expression of D14. OsMADS57 directly represses the expression of D14; OsTb2 interacts with OsTb1 to alleviate OsTb1’s inhibition on OsMADS57, which consequently reduces the expression of D14 and increases tillers.
