Fig. 8: Taccalonolide probes compared to commercial taxane-based probes. | Nature Communications

Fig. 8: Taccalonolide probes compared to commercial taxane-based probes.

From: Elucidating target specificity of the taccalonolide covalent microtubule stabilizers employing a combinatorial chemical approach

Fig. 8

a SK-OV-3 human ovarian cancer cells were treated with 0.5 µM of 11, Tubulin Tracker Green (PTX-OG; Thermo Fisher; middle), or siR-Tubulin (Cytoskeleton, Inc; bottom) for 5 h and imaged before treated media was removed (before wash), after the treated media were replaced by fresh medium (after wash), or after the treated cells were chilled at −20 °C for 20 min and fixed (chilled). b SK-OV-3-MDR1-M6/6 human ovarian cancer cells expressing P-glycoprotein were treated with 0.5 µM of each probe for 5 h and imaged at 37 °C before and after washing. The same image acquisition and visualization conditions were used for all images obtained for each probe. Brightfield images are shown in the far right column for each figure. Scale bars = 50 µm for all images. Source data are provided as a Source Data file.

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