Table 2 Antibodies used in this study.
Provider | Catalogue number | Conditions | |
|---|---|---|---|
Primary antibodies | |||
Alpha smooth muscle actin (1A4) | Sigma Aldrich | A2547 | 15 m Citrate. 1/2000 dilution O/N |
β-actin | Cell Signalling Technologies | 8457P | 1/5000 O/N (western blot) |
β-catenin | BD Transduction Laboratories | 610154 | 1 h Citrate 1/50 O/N |
Calnexin | Enzo | ADI-SPA-860-D | 1/2000 O/N |
CD45-PE | BioLegend | 103105 | 1 µl per 1 × 10e6 cells, 30 m RT |
CD11b-PECy7 | BioLegend | 101215 | 1 µl per 1 × 10e6 cells, 30 m RT |
CD68 (KP1) | Dako | M081401-2 | |
c-JUN (total) (60A8) | Cell Signalling Technologies | 9165 | 30 m Citrate. 1/100 dilution O/N 1/1000 O/N (western blot) |
Collagen-1 | Southern Biotech | 1310-01 | 5 m Citrate. 2/200 dilution 72 h |
Ctgf | Abcam | ab6992 | No antigen retrival 1/100 O/N |
Desmin (Y66) | Abcam | Ab32362 | 15 m Citrate. 1/100 dilution O/N |
E-cadherin DECMA-1 | Genetex | GTX11512 | 1/100 O/N |
GFP | Abcam | ab13970 | 5 m Tris-EDTA. 1/500 O/N |
Glutamine Synthatase | Abcam | ab64613 | 10 m Citrate 1/100 |
Keratin-19 (TROMA-III) | Developmental Studies Hybridoma Bank | Troma-III | 10 m Citrate. 1/200 dilution O/N |
pcJUN (ser73) (D47G9) | Cell Signalling Technologies | 3270 | 30 m Citrate. 1/100 dilution O/N |
pcJUN (ser63) (54B3) (western blot) | Cell Signalling Technologies | 2361 | 1/1000 O/N |
JNK (total) | Cell Signalling Technologies | 9252 | 30 m Citrate. 1/100 dilution O/N 1/1000 O/N (western blot) |
pJNK (Thr183/Tyr185) (81E11) | Cell Signalling Technologies | 4668 | 30 m Citrate. 1/100 dilution O/N |
PTK7 | Source Bioscience | LS-B10725 | 10 m Tris-EDTA 1/100 O/N |
RFP | Abcam | ab62341 | 10 m Citrate. 1/100 dilution O/N |
Vangl1 | Sigma Aldrich | HPA025235 | 10 m Tris-EDTA 1/100 O/N |
Vangl2 | Sigma Aldrich | HPA027043 | 10 m Tris-EDTA 1/100 O/N |
Vangl2 (western blot) | R&D Systems | AF4815 | 1/500 O/N |
Wnt5a | LS Bio | LS-B4565 | 10 m Tris-EDTA 1/100 O/N |
Secondary antibodies | |||
Anti-goat Alexa 594 | Thermo Fisher | A-27016 | 1/500 1 h RT |
Anti-goat Biotinylated | Vector Laboratories | BA-5000 | 1/500 1 h RT |
Anti-mouse Alexa 488 | Thermo Fisher | A32723 | 1/500 1 h RT |
Anti-rabbit Alexa 594 | Thermo Fisher | A-21207 | 1/500 1 h RT |
Anti-Rabbit Biotinylated | Vector Laboratories | BA-1000 | 1/500 1 h RT |
Anti-rabbit HRP | Vector Laboratories | PI-1000 | 1/500 1 h RT |
Anti-rat Alexa 488 | Thermo Fisher | A-21208 | 1/500 1 h RT |
Anti-rat Alexa 594 | Thermo Fisher | A-21209 | 1/500 1 h RT |
Tyramide Alexa 594 | Thermo Fisher | B40957 | 1/50 10 m RT |
Tyramide Alexa 488 | Thermo Fisher | B40953 | 1/50 10 m RT |
