Fig. 4: The antitumor CD8+ T cell response is augmented against IFN-γ-insensitive tumors. | Nature Communications

Fig. 4: The antitumor CD8+ T cell response is augmented against IFN-γ-insensitive tumors.

From: Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells

Fig. 4: The antitumor CD8+ T cell response is augmented against IFN-γ-insensitive tumors.

a Frequency of splenic IFN-γ-secreting T cells in response to SIY measured by enzyme-linked immunospot (ELISPOT). Splenocytes were isolated from mice on day 7 after tumor engraftment and cultured with 100 μM SIY for 18 h. n = 21 mice (WT), n = 20 mice (IFNγR2 Mut.1), and n = 10 mice (Jak1 Mut.1); data are pooled from 4 (IFNγR2 Mut.1) or 2 (Jak1 Mut.1) independent experiments. b Representative flow cytometry plots (left) and cumulative data (right) of H-2Kb/SIY+ CD8+ TILs isolated on day 7 after engraftment of WT, IFNγR2 Mut.1, and Jak1 Mut.1 tumor cells. n = 33 mice (WT), n = 34 mice (IFNγR2 Mut.1), and n = 18 mice (Jak1 Mut.1); data are pooled from six (IFNγR2 Mut.1) or four (Jak1 Mut.1) independent experiments. c As in b but with IFNγR2 Mut.1 tumors with reintroduced IFNγR2. n = 15 mice (WT and IFNγR2 Mut.1), n = 14 mice (IFNγR2 Mut.1 + IFNγR2); data are pooled from three independent experiments. d As in b but with Jak1 Mut.1 tumors with reintroduced Jak1. n = 17 mice (WT), n = 13 mice (Jak1 Mut.1), and n = 10 mice (Jak1 Mut.1 + Jak1); data are pooled from three independent experiments. Results are expressed as mean ± s.e.m. Statistical significance was determined by a Kruskal-Wallis (non-parametric) test (a–d). *p < 0.05, **p < 0.01, ***p < 0.001.

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