Fig. 3: The 6-bp deletion in the fifth exon of WTK3 is critical for the powdery mildew resistance of Pm24. | Nature Communications

Fig. 3: The 6-bp deletion in the fifth exon of WTK3 is critical for the powdery mildew resistance of Pm24.

From: A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

Fig. 3: The 6-bp deletion in the fifth exon of WTK3 is critical for the powdery mildew resistance of Pm24.

a The structures of the main transcript of WTK3 in S4185, and the constructs of WTK3 overexpression variants ProUbi:WTK3HLT, ProUbi:WTK3#1, ProUbi:WTK3#2, and ProUbi:WTK3#3 used for transgenic assay. Ubi, promoter of the maize polyubiquitin gene. LB, left border; RB, right border. b Infection reactions of Fielder (IT 4) and the T1 transgenic plants of ProUbi:WTK3HLT (IT 0), ProUbi:WTK3#1 (IT 4), ProUbi:WTK3#2 (IT 4), and ProUbi:WTK3#3 (IT 4) inoculated with Bgt isolate E09. Three representative individuals of Fielder and each transgenic line were photographed at 10 dpi; + /−: presence/absence of the transgene.

Back to article page