Fig. 1: Sequence and structure of LUXendin555, LUXendin645, and LUXendin651. | Nature Communications

Fig. 1: Sequence and structure of LUXendin555, LUXendin645, and LUXendin651.

From: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Fig. 1: Sequence and structure of LUXendin555, LUXendin645, and LUXendin651.The alternative text for this image may have been generated using AI.

LUXendins are based on the antagonist Exendin4(9–39), shown in complex with GLP1R. The label can be any dye, such as TMR (top), SiR (middle), or Cy5 (bottom) to give LUXendin555, LUXendin645, and LUXendin651, respectively. The model was obtained by using the cryo-EM structure of the activated form of GLP1R in complex with a G protein (pdb: 5VAI)62, with the G protein and the 8 N-terminal amino acids of the ligand removed from the structure while mutating S39C and adding the respective linker. Models were obtained as representative cartoons by the in-built building capability of PyMOL (Palo Alto, CA, USA) without energy optimization. Succinimide stereochemistry is unknown and neglected for clarity.

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