Fig. 4: Surface charge of the GluN1 NTD hinge region affects NMDAR mobility in mature neurons. | Nature Communications

Fig. 4: Surface charge of the GluN1 NTD hinge region affects NMDAR mobility in mature neurons.

From: Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility

Fig. 4

a Representative FRAP images at different time points of DIV21–23 cortical neurons transfected with EGFP–GluN1 (WT, Quintuple, or Sextuple). Recovery of bleached spine puncta (magenta circle) was monitored for 30 min at 30-s intervals. Scale bar = 2 µm. b Quantification of the recovery curve of different GluN1 mutants in DIV21–23 cortical neurons. Graphs represent mean intensity and show fit (****p < 0.0001, Kolmogorov–Smirnov (KS) nonparametric test). Inset: Quantification of the mobile fraction of EGFP–GluN1 puncta in dendritic spines at 30 min after  photobleaching in EGFP–GluN1 mutant transfected cells compared to WT EGFP–GluN1 in DIV21–23 (*p < 0.05, ANOVA; green dots represent WT n = 14 puncta; Quintuple n = 11; Sextuple n = 10). Error bars show S.E.M.

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