Fig. 1: The Biopsy Trifecta EXTraction based proteogenomics workflow.
From: Microscaled proteogenomic methods for precision oncology
a In the Biopsy Trifecta EXTraction (BioTEXT) protocol, patient derived OCT-embedded core needlebiopsies are sectioned, followed up by extraction of DNA, RNA and proteins for deep-scale proteogenomics characterization and by immunohistochemistry-based imaging. b The Microscaled Proteomics (MiProt) workflow allows deep-scale proteomics and phosphoproteomics characterization with 25 μg of peptides per core-needle biopsy. MiProt uses a common reference that could be used for comparison across all samples within a single-TMT10/11 plex and across several TMT10/11 plexes spanning several core biopsies.
