Fig. 2: OGDs tailor the purification and native MS analysis of membrane proteins. | Nature Communications

Fig. 2: OGDs tailor the purification and native MS analysis of membrane proteins.

From: Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Fig. 2

a Three membrane proteins were isolated from cell membranes using OGDs 1–5. b Higher relative protein concentrations are obtained from [G1] OGDs. The [G1] OGD regioisomer mixture 2 (=2a + 2b) leads to a higher extraction yield than the individual regioisomers 2a and 2b. Relative protein concentrations were plotted with standard deviation ( ± s.d., n = 3). c Mass spectra obtained from tetrameric AqpZ-GFP after isolation with the [G1] OGD regioisomer mixture 1 reveal no protein complexes with native membrane lipids (upper spectrum). Substitution of the head group and hydrophobic tail in the [G2] OGD regioisomer mixture 4 enabled the detection of protein complexes with native membrane lipids (lower spectrum). Spectra were acquired using similar instrument conditions (HCD energy: 200 V). AqpZ tetramers are indicated by four blue circles, cardiolipins by violet circles, and phospholipids by orange circles. Source data are provided as a Source Data file.

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