Fig. 7: Covisualization of optically controlled iB(actin) and its endogenous target. | Nature Communications

Fig. 7: Covisualization of optically controlled iB(actin) and its endogenous target.

From: Optogenetic regulation of endogenous proteins

Fig. 7

a HeLa cells expressing the optically controlled iB(RAS) (shown in red) were stained with anti-pan-RAS monoclonal antibodies (green). iB(RAS) has nuclear localization in darkness. Under 740 nm light, it is released from nucleus to cytoplasm and plasma membrane where it is colocalized with endogenous RAS. Zoom in of white frame-marked regions are shown on the right. Epifluorescence microscopy; scale bar, 10 µm. b Cells with the NIR light-triggered release of iB(RAS) imaged using confocal microscopy. Scale bar, 10 µm.

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