Fig. 2: Base editors with C-terminally truncated A3A sequences exhibit narrowed editing windows.
From: Expanding the genome-targeting scope and the site selectivity of high-precision base editors
a Structure of A3A-BE3 and BEs with A3A truncations (A3AΔ-BE3 variants). The various A3A truncations tested are shown in Supplementary Fig. 11. b, c Effects of C terminal truncations of the A3A domain on the width of the editing window of A3AΔ-BE3s. All base editor variants were tested on both the polyC-7 (b) and polyC-8 (c) sites (see “Methods” section). Cs within each target region are indicated in red, with the number below indicating their distance from the PAM (blue). The C-to-T conversion efficiencies are plotted for all Cs within the protospacer, and shown in comparison to the A3A-BE3 base editor with the full-length A3A (gray bars). Values and error bars represent the mean and standard deviation of three biological replicates. Source data underlying b, c are provided as a Source Data file.
