Fig. 3: Base editing outcomes of A3A-BE3, truncated A3AΔ-BE3 variants and the recently optimized editor eA3A-BE3 (ref. 22) when targeting specific sites in the yeast Can1 gene.
From: Expanding the genome-targeting scope and the site selectivity of high-precision base editors
a Sequences of the five target sites (containing Cs at different positions). Target Cs are indicated in red and numbered relative to the PAM (blue). Edited clones were identified by using the canavanine selection strategy (see “Methods” section). b Base editing efficiency and precision. The x-axis represents the target Cs within the protospacers. The y-axis shows their C-to-T mutation frequency (see “Methods” section). Values and error bars represent the mean and standard deviation of three independent biological replicates. Source data underlying b are provided as a Source Data file.
