Fig. 4: Analysis of off-target editing. Genetic changes that occurred in strains harboring nCDA1-BE3, cCDA1-BE3, nCDA1Δ190-BE3 or a control plasmid without a BE construct were identified by whole-genome sequencing.
From: Expanding the genome-targeting scope and the site selectivity of high-precision base editors
a, b Comparison of the total number of detected indels (a) and SNVs (b). For information on the constructs used and the experimental workflow, see Supplementary Fig. 13. c The mutation frequency of different types of SNVs in cells treated by the three base editors and the control. The sgRNA was designed to target site Can1-4 (Supplementary Table 1). Values and error bars represent the mean and standard deviation of three independent biological replicates. Source data are provided as a Source Data file.
