Table 1 Recommendations for BE selection for precision cytosine base editing.
From: Expanding the genome-targeting scope and the site selectivity of high-precision base editors
Distance of target C from PAM | Bystander | Recommended BEs |
|---|---|---|
<−19 | no | nCDA1-BE3, nCDA1Δ198-BE3, A3A-NL-BE3 |
−19 | no | nCDA1-BE3, nCDA1Δ198-BE3, A3A-NL-BE3 |
CCDDD | cCDA1-BE3 | |
−18 | no | nCDA1Δ198-BE3, n/cCDA1-BE3, A3A-NL-BE3 |
NCN | nCDA1Δ(194-188)-BE3 | |
−17 | no | nCDA1Δ198-BE3, cCDA1-BE3, A3A-BE3, nCDA1-BE3, BE3 |
DCN | nCDA1Δ(194-188)-BE3 | |
CCDDDD | BE3 | |
−16 | no | BE3, nCDA1Δ198-BE3, A3A-BE3 |
DDDCC | cCDA1-BE3 | |
TCC | cCDA1-BE3, YEE-BE3, BE-PAPAPAP | |
NCD | A3AΔ182-BE3, A3A(Y130F)Δ186-BE3 | |
−15 | no | BE3, nCDA1Δ198-BE3, A3A-BE3 |
CDCD | BE-PAPAPAP, A3AΔ182-BE3, A3A(Y130F)Δ186-BE3, YEE-BE3 | |
DCN | A3AΔ182-BE3, A3A(Y130F)Δ186-BE3 | |
RCCD | BE-PAPAPAP | |
−14 | no | BE3, A3A-BE3, nCDA1-BE3 |
DDCC | BE-PAPAPAP | |
>−14 | no | A3A-BE3, nCDA1-BE3, BE3 |
