Fig. 5: NKTR-214 induces intratumoral production of IFN-γ and TNF-α effector cytokines. | Nature Communications

Fig. 5: NKTR-214 induces intratumoral production of IFN-γ and TNF-α effector cytokines.

From: Bempegaldesleukin selectively depletes intratumoral Tregs and potentiates T cell-mediated cancer therapy

Fig. 5

a C57BL/6 mice bearing 7-day-old, s.c. B16.F10 tumors received pmel-1 T cells followed by treatment with NKTR-214 plus gp100 vaccination. Absolute numbers of pmel-1 Teff and Tregs in tumor and spleen on indicated days are plotted. Correlation between Teff and Treg numbers through time in each tissue was calculated, and Pearson’s and Spearman correlation coefficients are shown. b Level of IFN-γ and TNF-α protein in tumors of untreated or vaccine-NKTR-214-treated mice on day 7. Data represented as mean ± SEM (n = 5, ****P < 0.0001, unpaired t test). c Gene expression profiling of IFN-γ and TNF-α performed via NanoString in tumor biopsies from melanoma and renal cell carcinoma patients (n = 8), pre (−) and week 3 post NKTR-214 treatment (+). RNA counts are represented as mean ± SEM (*P < 0.05 ratio, paired t test).

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