Fig. 6: CD8+ Teff-derived IFN-γ and TNF-α synergize to deplete intratumoral Tregs. | Nature Communications

Fig. 6: CD8+ Teff-derived IFN-γ and TNF-α synergize to deplete intratumoral Tregs.

From: Bempegaldesleukin selectively depletes intratumoral Tregs and potentiates T cell-mediated cancer therapy

Fig. 6

a–d C57BL/6 mice bearing 7-day-old, s.c. B16.F10 tumors received pmel-1 T cells, and were either left untreated or received gp100 peptide vaccine and/or NKTR-214. Where indicated, mice received neutralizing antibodies against IFN-γ and TNF-α on days 1, 2, 4, and 6. a Tregs in tumor (upper panel) and spleen (lower panel). b Expression of Ki67 and c Annexin V on intratumoral Tregs on day 7 post treatment. d Heat map depicting protein level of indicated cytokines and chemokines in tumor and spleen of mice 7 days after treatment. Color scale denotes levels of cytokines/chemokines in pg/ml. Data represented as mean ± SEM (n = 5, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns nonsignificant, unpaired t test).

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