Fig. 4: Dnmt3a2 overexpressing neuronal ensembles enhanced stability is context-specific.
From: Neuronal ensemble-specific DNA methylation strengthens engram stability
a Experimental schedule used to assess the engram stability at memory recall in a novel context. b Representative images of immunohistochemical analysis of myc-GFP and Fos in mice injected with RAM-myc-GFP and E-SARE-HA-LacZ or E-SARE-HA-Dnmt3a2. Scale bar represents 50 μm. c Observed over chance overlap between myc-GFP and Fos expressions in mice injected with RAM-myc-GFP and E-SARE-HA-LacZ or E-SARE-HA-Dnmt3a2 (Observed overlap vs chance overlap: HA-LacZ: n = 5, t(4) = 2.605, p = 0.0597 by paired t-test; HA-Dnmt3a2: n = 7, W = 12, p = 0.3750 by Wilcoxon test; Observed/chance HA-LacZ (n = 5) vs HA-Dnmt3a2 (n = 7), t(10) = 0.797, p = 0.4440 by unpaired t-test). Dashed line represents the chance level. d Analysis of the reactivation of the learning-activated neuronal population during memory recall in an altered context in mice injected with RAM-myc-GFP and E-SARE-HA-LacZ or E-SARE-HA-Dnmt3a2 (HA-LacZ (n = 5) vs HA-Dnmt3a2 (n = 7), t(10) = 0.4158, p = 0.6864 by unpaired t-test). e Percentage of GFP and Fos positive neurons after memory recall in an altered context in mice injected with RAM-myc-GFP and E-SARE-HA-LacZ or E-SARE-HA-Dnmt3a2 (HA-LacZ (n = 5) vs HA-Dnmt3a2 (n = 7), t(10) = 0.8695, p = 0.4050 by unpaired t-test). RR: Reactivation rate; w- week, DG: dentate gyrus of the hippocampus, CFC: contextual fear conditioning, Dox: doxycycline, rAAVs: recombinant adeno-associated viruses. ns: not significant by the respective statistical test. Error bars represent s.e.m. Source data are provided as a Source Data file.
