Fig. 5: Dnmt3a2 alters the DNA methylation profile of memory-related genes.
From: Neuronal ensemble-specific DNA methylation strengthens engram stability
a Experimental design used to identify DNA methylation changes in response to Dnmt3a2 overexpression in primary hippocampal cultures. b Number of hyper- (higher methylation in HA-Dnmt3a2-expressing cells) and hypo- (lower methylation in HA-Dnmt3a2-expressing cells) DMRs identified by whole-genome DNA methylation profiling. N = 3 independent cell preparations per condition (ESARE-HA-GFP versus ESARE-HA-Dnmt3a2). c Genomic distribution of DMRs with respect to the gene model. d Top GO categories found enriched using the Metascape analysis of genes in the vicinity of hyper-DMRs. Shown are only categories with adjusted P > 0.01 (hypergeometric test, Benjamini–Hochberg adjustment). DIV: day in vitro, WGBS: whole-genome bisulfite sequencing, DMR: differentially methylated regions, rAAV: recombinant adeno-associated virus, UTR: untranslated region.
