Fig. 5: Parkin-dependent recruitment of TBC1D9 to depolarized mitochondria involves Ca2+ signaling.
From: TBC1D9 regulates TBK1 activation through Ca2+ signaling in selective autophagy
a HeLa cells expressing mCherry-Parkin were treated with AO for 4 h and stained for TBC1D9 and TOM20. Scale bars, 10 μm. b, c HeLa cells expressing EmGFP-TBC1D9 and mCherry-Parkin were treated with AO for 4 h and stained for TOM20. b Representative confocal images and c Pearson’s coefficient between TBC1D9 and TOM20. Data in (c) represent the mean ± SEM of three independent experiments (n > 5 images per condition). d HeLa cells expressing mCherry-Parkin and EmGFP-TBC1D9 (WT, ΔEF-hand motif, and ΔUBR) were treated with AO for 4 h and stained for TOM20. e HeLa cells expressing EmGFP-TBC1D9 and mCherry-Parkin were treated with AO and BAPTA-AM for 4 h and stained for TOM20. P values calculated by two-tailed Student’s t test.
