Fig. 1: A secreted factor of B. cereus activates the inflammasome independently of HBL. | Nature Communications

Fig. 1: A secreted factor of B. cereus activates the inflammasome independently of HBL.

From: Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome

Fig. 1

a Immunoblot analysis of pro-caspase-1 (Casp-1), the active caspase-1 p20 subunit, pro-pyroptotic effector protein gasdermin D (GSDMD), the active GSDMD p30 subunit of WT BMDMs left untreated [Medium alone (Med.)] or LPS-primed and assessed either 3 h or 20 h after stimulation with the supernatant of WT B. cereus (B. cer.) or ΔHbl B. cereus (ΔHbl) (top; Supernatant (Sup.)) or infection with B. cer. or ΔHbl (bottom; m.o.i. of 5). Release of IL-1β b, IL-18 c, and cell death d of BMDMs as treated in a. NS, not significant, ***P < 0.001 and ****P < 0.0001 (student’s unpaired t test b–d). Each symbol represents an independent experiment b–d. Data are representative of three independent experiments (n = 3, a–d; mean and s.e.m. in b–d). Source data are provided as a Source Data file.

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