Fig. 10: NHE induces activation of the NLRP3 inflammasome in the host.

a Concentration of IL-18 in the serum of WT mice assessed 16 h after intraperitoneal injection with 200 μl of supernatant of WT B. cereus (Sup.), which had been treated with either an isotype control, anti-HBL-neutralizing antibodies (α-HBL), anti-NHE-neutralizing antibodies (α-NHE), or both (α-Tox.). b Concentration of IL-18 in the peritoneal fluid of WT mice assessed 1 h after intraperitoneal injection with treatment as in a. c Concentration of IL-18 in the serum of WT mice assessed 16 h after intraperitoneal injection with 200 μl ΔHbl B. cereus supernatant, which had been treated with either an isotype control or anti-NHE-neutralizing antibodies (α-NHE). d Concentration of IL-18 in the peritoneal fluid of WT mice assessed 1 h after intraperitoneal injection with treatment as in c. e Concentration of IL-18 in the serum of WT, Nlrp3^−/−, Casp1/11^−/− or Gsdmd^I105N/I105N mice assessed 6 h after intraperitoneal injection with 5 × 10⁶ CFU of ΔHbl B. cereus (ΔHbl). f Concentration of IL-18 in the peritoneal fluid of WT, Nlrp3^−/−, Casp1/11^−/−, or Gsdmd^I105N/I105N mice assessed 6 h after intraperitoneal injection with treatment as in e. g Concentration of IL-18 in the serum of WT mice administered with either PBS or MCC950, both intraperitoneally, followed by intraperitoneal infection with 5 × 10⁶ CFU of ΔHbl with a corresponding second dose of PBS or MCC950, 6 h after infection. h Concentration of IL-18 in the peritoneal fluid of WT mice administered with either PBS or MCC950 (as in g) 6 h after infection with 5 × 10⁶ CFU of ΔHbl. **P < 0.01, ***P < 0.001, and ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple-comparisons test a, b, e, and f or Student’s unpaired t test c, d, g, and h. Each symbol represents an individual mouse a–h. Data are pooled from two independent experiments (n = 2 in a–h; mean and s.e.m. in a–h). Source data are provided as a Source Data file.