Fig. 3: Neutralization of both HBL and NHE abrogates inflammasome activation.

a Immunoblot analysis of caspase-1 and gasdermin D, of WT BMDMs left untreated or LPS-primed and assessed 20 h after treatment with the supernatant of WT B. cereus (Sup.) treated with an isotype control (Iso.), anti-HBL-neutralizing antibodies (α-HBL), anti-NHE-neutralizing antibodies (α-NHE) or with both anti-HBL- and anti-NHE-neutralizing antibodies (α-Tox.). Release of IL-1β b, and IL-18 c of BMDMs, death of BMDMs d, and release of TNF e, of BMDMs as treated in a. f Immunoblot analysis of caspase-1 and gasdermin D of WT BMDMs left untreated or LPS-primed and assessed 20 h after treatment with the supernatant of ΔHbl treated with an isotype control or anti-NHE-neutralizing antibodies or assessed 20 h after treatment with the supernatant of ΔNhe B. cereus (ΔNhe Sup.) treated with an isotype control or anti-HBL-neutralizing antibodies. Release of IL-1β g, and IL-18 h of BMDMs, death of BMDMs i, and release of TNF j of BMDMs as treated in f. NS, not significant, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple-comparisons test b–e or student’s unpaired t test g–j. Each symbol represents an independent experiment b–e and g–j. Data are representative of three independent experiments (n = 3 in a–j mean and s.e.m. in b–e, and g–j). Source data are provided as a Source Data file.