Fig. 8: The transmembrane domain in NHE-C is essential for inflammasome activation and cell death.
From: Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome
a Immunoblot analysis of caspase-1 of WT or Nlrp3−/− BMDMs left untreated (Med.) or LPS-primed and assessed 3 h after treatment with C + B + A (NHEWT) or Cmut. + B + A (NHEmut.). b Release of IL-1β and IL-18, and death of WT or Nlrp3−/− BMDMs as treated in a. c Inductively coupled plasma-optical emission spectrometry analysis of intracellular concentrations of K+ of BMDMs left untreated or assessed 3 h after treatment with NHEWT or NHEmut.. d Circular dichroism analysis of the secondary structures of CWT or Cmut.. e IncuCyte live-imaging analysis of the viability of WT BMDMs left untreated or LPS-primed and assessed after treatment with NHEWT or NHEmut.. f Scanning electron microscopy analysis of WT BMDMs left untreated or LPS-primed and assessed 3 h after treatment with NHEWT or NHEmut.. Scale bar, 2 μm f. NS, not significant, ***P < 0.001 and ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple-comparisons test b, c or Student’s unpaired t test e). Each symbol represents an independent experiment b, c, and e. Data are representative of three independent experiments (n = 3 in a–c, e, and f; n = 1 in d; mean and s.e.m. in b, c, and e. Source data are provided as a Source Data file.
