Fig. 9: The transmembrane domain in NHE-C mediates membrane binding and pore formation.
From: Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome
a Immunoblot analysis of CD14, caspase-1, C, B, and A of unprimed WT BMDMs left untreated or assessed 1 h after treatment with NHEWT or NHEmut.. Mem, membrane fraction; Cyto, cytosolic fraction. b Immunofluorescent analysis of CD11b (green) and C (red) in unprimed WT BMDMs left untreated or assessed 1 h after treatment with CWT or Cmut.. c Colorimetric analysis (top) and the absorbance (OD, bottom) of liposomes left untreated, sonicated for 5 mins at 100 amplitude (CTRL) or assessed 30 mins after treatment with NHEWT or NHEmut.. d Cryo-transmission electron microscopy analysis of liposomes assessed 1 h after treatment with NHEWT (n = 451) or NHEmut. (n = 545). Quantification of membrane pores of liposomes (right). Arrowheads indicate pores d. Scale bar, 25 μm b, 50 nm d. NS, not significant, **P < 0.01, ***P < 0.001 (one-way ANOVA with Dunnett’s multiple-comparisons test c or Student’s unpaired t test d). Each symbol represents an independent experiment c, d. Data are representative of three independent experiments (n = 3 in a–d; mean and s.e.m. in c, d). Source data are provided as a Source Data file.
