Fig. 1: DSB-indicating Rad52 forms liquid droplets in vivo.
a–c Live-cell microscopy shows that nuclei with >1 Rad52 foci (a) exhibit shorter microtubule filaments (b) that cannot capture the foci (c) (n = 3). In a, shown are percentage of cells with given DIM species and pti-DIM-indicating blue arrowheads. d, e Rad52 foci exhibit liquid droplet behaviour (n = 6). White arrowheads highlight relevant behaviours. f Rad52 foci dynamically exchange constituents with the nucleoplasm (Rad52 n = 30, Tub1 n = 12). Scale bars, 1 µm. Quantifications represent the mean ± s.d.; *P < 0.0001 in Mann–Whitney U (b) and χ2 (c) tests. Source data are provided as a Source Data file.
