Fig. 5: Rad52 repair centre droplets concentrate tubulin and project filaments for perinuclear DSB targeting. | Nature Communications

Fig. 5: Rad52 repair centre droplets concentrate tubulin and project filaments for perinuclear DSB targeting.

From: DNA repair by Rad52 liquid droplets

Fig. 5

a, b Live-cell confocal microscopy and quantification showing that Rad52 droplets exceeding 0.2 µm2 in size (red line) have a 10-fold higher chance of concentrating tubulin (n = 6, 168 droplets). c–e Tubulin foci inside Rad52 droplets project aster-DIMs (c) that reach the long DIM (d) before stable Rad52 droplet capture by the DIM (e). Frequency of events is shown in white. MTs microtubules. f Proposed model. Quantifications represent the mean ± s.d.; *P ≤ 0.0001 in Mann–Whitney U tests. n = 77 (Tubulin-) and 46 droplets (Tubulin+) over six independent experiments (b). Scale bars, 1 µm. Source data are provided as a Source Data file.

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