Fig. 2: Competitive dynamic covalent assembly.
From: Sequence-selective dynamic covalent assembly of information-bearing oligomers
a MALDI mass spectra of reaction mixtures generated after applying the dissociation/extraction/annealing process to non-complementary 01010 and 10001 sequences (bottom, red) and to a ternary mixture of a competitive environment consisting of a complementary sequence and a non-complementary sequence via the dissociation/extraction/annealing process (top, blue). Peaks at multiples of +18m/z values are attributable to ladders species with fewer rungs. b Sequence-selective hybridization assessed by FRET and normalized to the fluorescence intensity of a 01010-FAM sequence single strand. Assembly solutions were excited at 495 nm and fluorescence emission was measured at 425 nm. Error bars represent standard error. The horizontal solid and dashed lines 1 and 0.2, respectively, represent the unquenched 01010-FAM control and the reported FAM/DABCYL fluorescence quenching efficiency of 80%40, respectively.
