Fig. 4: Loss of Men1 triggers aberrant DNA damage repair. | Nature Communications

Fig. 4: Loss of Men1 triggers aberrant DNA damage repair.

From: MEN1 deficiency leads to neuroendocrine differentiation of lung cancer and disrupts the DNA damage response

Fig. 4

a, b IHC staining for γH2AX was performed in lung tissue/tumor sections from mice of the indicated genotypes. a The WT, MS, KS, and KMS 11 weeks mice lung tissues. bThe Men1+/− 18 months mice lung tissues and the Men1Δ/Δ 12 months mice lung tissues. Scale bars, 20 μm. c Representative images of DAPI staining showing NBUDs, NPBs, and MNi (red arrows) in Men1f/f and Men1Δ/Δ MEF cells 1 hour after exposure to 10 Gy of IR. The statistical results were obtained from analysis of mitotic cells(n = 100 per group), and repeat the count three times. Scale bars, 10 μm. d IF staining for γH2AX (red), menin (green), and DAPI (blue) in p53f/f and p53Δ/Δ MEF cells 1 hour after exposure to the indicated IR dose. Colocalization of menin and γH2AX is shown in gray. Scale bars, 10 μm. e Western blotting was used to detect the expression of the indicated proteins in A549 and MEF cells 1 hour after exposure to 10 Gy of IR. Data are represented as mean ± SD in c. Significance determined by two-tailed unpaired t tests in c are indicated. Results in c, d, and e are representative of three independent experiments. Source Data are provided as a Source Data file.

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