Fig. 1: ADAR proteins regulate a subset of alternative splicing events.

a WB analyses of ADAR1 and ADAR2 proteins in EC109 cells that were stably knocked down (shADAR1 #3 and #9; shADAR2 #939 and #942; and scramble shRNA (scr)) or overexpressed (pLenti-ADAR1; pLenti-ADAR2; and empty vector control) for ADAR1 or 2, using lentiviral system. β-actin (actin) was used as a loading control. b Pie charts representing the number (and percentage) of each type of alternative splicing events affected by ADAR1 (left) and ADAR2 (right). c Heat maps showing the differentially spliced cassette exon events, upon knockdown and overexpression of ADAR1 (left) and ADAR2 (right). Splicing index (SI) is calculated by the ratio of inclusion junction reads to the sum of inclusion and skipping junction reads, and ΔSI indicates the difference in SI between ADARs knockdown/overexpression and their corresponding control samples. d, e RT-PCR analyses of representative ADAR1- d or ADAR2- e affected cassette exons in original RNA-Seq EC109 cells, as well as HEK293T cells. PSI, percent spliced in. Source data are provided as a Source Data file.