Fig. 6: Embryonic silencing of miR-1-3p leads to incomplete feminization.

a Relative expression levels of miR-1-3p transcript in embryos 10 h and 20 h after injection of the miR-1-3p antagomir, (b) relative expression levels of Bdtra transcript in embryos 10 h and 20 h after injection of the miR-1-3p antagomir, (c) dissected normal ovaries from a female injected with the antagomir negtive control (NC), and (d) dissected partially feminized ovaries from a female injected with the miR-1-3p antagomir. Error bars indicate the SEM of three independent biological replicates and asterisks (**) indicate the statistically significant differences (P < 0.01) between the treatment group and control group based on Student’s t test.