Fig. 5: CDK8 subcomplex chromatin occupancy correlates with changes in enhancer acetylation. | Nature Communications

Fig. 5: CDK8 subcomplex chromatin occupancy correlates with changes in enhancer acetylation.

From: Altered chromatin landscape and enhancer engagement underlie transcriptional dysregulation in MED12 mutant uterine leiomyomas

Fig. 5

a Heat maps of normalized CDK8 and MED12 ChIP-seq reads at differentially bound sites in myometrium vs. leiomyoma (left panel). Heat maps represent average signal of five biological replicates. Right panel shows box and whisker plot of normalized reads at CDK8 and MED12 co-bound sites that show enrichment or depletion of both factors. Plots of sites with depleted (−) and enriched (+) CDK8 submodule signal are shown separately. Centerline represents the median, bounds of box represent the interquartile range (IQR) and whiskers are 1.5 × IQR. b, c Scatter plots of fold change in H3K27Ac signal against fold change in CDK8 (b) and MED12 (c) at enhancers with enriched or depleted CDK8 and MED12 binding. R represents the Pearson’s correlation coefficient. d, e Tag density plots of CDK8, MED12, JUN, and FOS ChIP-seq enrichment profiles at FOS (d) and JUN (e) enhancer binding sites in myometrium and leiomyoma. f, g AP-1 subunits (JUN and FOS) and CDK8 submodule (CDK8 and MED12) binding profiles at FN1 (f) and ETS2 (g) associated genomic loci. Myometrium and leiomyoma enhancer-promoter contacts and RNA-seq genomic tracks are also shown.

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